Recombinant Mouse Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Lys141 is expressed.
Greater than 98% as determined by reducing SDS-PAGE.
内毒素水平
Less than 0.03EU/μg as determined by LAL test.
活性
Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is 0.1 - 0.3 ng/mL.
溶解方法
Recombinant Mouse GM-CSF/CSF2 Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. It is recommended to dissolve and dilute with a complete medium containing 10% calf serum when used for cell culture.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
储存/保存方法
Recombinant Mouse GM-CSF/CSF2 Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
靶标
背景说明
Granulocyte-macrophage colony-stimulating factor is an enzyme that in mouse is encoded by the Csf2 gene, belongs to the GM-CSF family.CSF2 is a Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes