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| 品牌 | absin | 供货周期 | 两周 |
|---|
| 概述 | |
|---|---|
| 别名 | Beta Actin; beta-Actin; ACTB; Actin cytoplasmic 1; Actin, beta; Beta actin; beta cytoskeletal actin;A X actin like protein; ACTB; Actin cytoplasmic 1; alpha sarcomeric Actin; Actx; Beta cytoskeletal actin; Melanoma X actin; PS1TP5BP1. |
| 克隆类别 | Polyclonal |
| 同种亚型 | IgG |
| 反应种属
| Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep , Bee, Fish, Guinea Pig, Hamster, Goat, Cat, mt,op |
| 来源宿主 | Rabbit |
| 应用
| WB,ELISA,IHC-P,IHC-F,Flow-Cyt,IF |
| 形态 | Lyophilized or Liquid |
| 特异性 | beta-Actin (Loading Control) |
| 使用方法 | WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性能 | |
|---|---|
| 生产说明 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 背景 | Loading Control This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]. |
| 保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 浓度 | 1mg/1ml |
| 研究领域 | 肿瘤；细胞生物；信号转导；细胞骨架； |
| Gene IDs | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 分子量 | 42kDa |
| 功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
实验结果图
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-actin at 1/500 dilution
Lane2: Anti-beta-actin at 1/1000 dilution
Lane3: Anti-beta-actin at 1/2000 dilution
Lane4: Anti-beta-actin at 1/4000 dilution
Lane5: Anti-beta-actin at 1/5000 dilution
Lane6: Anti-beta-actin at 1/8000 dilution
Lane7: Anti-beta-actin at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 47 kD
Sample:
A549 Cell (Human) Lysate at 30 ug
Primary: Lane1: Anti-beta-actin at 1/500 dilution
Lane2: Anti-beta-actin at 1/1000 dilution
Lane3: Anti-beta-actin at 1/2000 dilution
Lane4: Anti-beta-actin at 1/4000 dilution
Lane5: Anti-beta-actin at 1/5000 dilution
Lane6: Anti-beta-actin at 1/8000 dilution
Lane7: Anti-beta-actin at 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 47 kD
Sample:
Lane1: 293T Cell Lysate at 25 ug
Lane2: A549 Cell Lysate at 25 ug
Lane3: A431 Cell Lysate at 25 ug
Primary: Anti- beta-Actin at 1/1000 and 1/5000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42 kD
Sample: Lung lysate at 30ug;
Primary: Anti-beta-actin at 1:1000 dilution
Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:3000 dilution
Predicted band size : 42kD
Observed band size : 42kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-Beta-actin Polyclonal Antibody, Unconjugated secondary primary antibody 1:1500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Blank control: RSC96(blue).
Primary Antibody: Rabbit Anti-beta-Actin /FITC Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody for 30 min on ice. Acquisition of 20,000 events was performed.
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