Recombinant Rhesus Macaque GM-CSF（2ug）

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产品型号：abs00962
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更新时间：2024-07-08
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细胞生物学

重组蛋白原代细胞分离细胞培养细胞检测细胞/组织处理外泌体研究其它动物血清 3D/类器官培养

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详细介绍

品牌	absin	货号	abs00962
规格	2ug	供货周期	一周

概述
种属	Rhesus Macaque
形态	Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
来源	Escherichia coli.
氨基酸序列	APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE
内毒素水平	Less than 1 EU/μg of rRhGM-CSF as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁷ IU/mg.
Accession #	Q9GL44
预期分子量	Approximay 14.4 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度	> 98 % by SDS-PAGE and HPLC analyses.

参考文献
1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3. 2. 1989. N Engl J Med, 320: 253-4. 3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101. 4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27. 5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.

参考文献

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.

2. 1989. N Engl J Med, 320: 253-4.

3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.

4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.

5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.

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Recombinant Rhesus Macaque GM-CSF（2ug）

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