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| 品牌 | absin | 货号 | abs04194 |
|---|---|---|---|
| 规格 | 10ug | 供货周期 | 一周 |
| 概述 | |
|---|---|
| 描述 | Recombinant Human Ephrin-B2 is produced by our Mammalian expression system and the target gene encoding Ile28-Ala229 is expressed with a 6His tag at the C-terminus. |
| 别名 | Ephrin-B2, EPH-Related Receptor Tyrosine Kinase Ligand 5, LERK-5, HTK Ligand, HTK-L, EFNB2, EPLG5, HTKL, LERK5 |
| 形态 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| 来源 | Human Cells |
| 氨基酸序列 | IVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIK KENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRA MKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILG SEVALFAVDHHHHHH |
| 内毒素水平 | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Accession # | P52799 |
| 性能 | |
|---|---|
| 背景 | Ephrin-B2 is a type I transmembrane protein and belongs the Ephrin family. It binds to the receptor tyrosine kinases, such as EPHA4, EPHB4 and EPHA3. Ephrin-B2 has been implicated in mediating developmental events, especially in the nervous system, erythropoiesis and tumour metastasis. Ligation of Ephrin-B2 with complementary EphB receptors on adjacent cells results in a combination of forward (EphB receptors) and reverse (Ephrin-B2) signalling, which is central to tissue development and remodelling functions. In addition, Ephrin-B2 may play a role in constraining the orientation of longitudinally projecting axons. |
| 溶解方法 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 保存方法 | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| 纯度 | Greater than 95% as determined by reducing SDS-PAGE. |
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