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品牌 | absin | 供货周期 | 现货 |
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应用领域 | 化工,生物产业,能源 |
概述 | |
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特异性 | |
反应种属 | |
预测反应种属 | |
应用 | |
免疫原 | |
性能 | |
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纯化方法 | |
类型 | |
同种型 | |
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存储溶液 | |
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背景说明 | |
细胞定位 | |
UniProt | |
实验结果图 | |
Sample: Raw246.7 (Mouse)Cell Lysate at 40 ug Primary: Anti- IL-1 beta (abs120224) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 30 kD | |
Tissue/cell: Human cervical carcinoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-IL-1 Beta/IL-1B Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining | |
Blank control (black line): A431(black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary antibody: Rabbit Anti-PTP1B antibody ;Dilution: 1μg /10^6 cells; Isotype Control antibody: Rabbit IgG . Secondary antibody: Goat anti-rabbit IgG-FITC;Dilution: 1μg /test. | |
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