Recombinant Human bFGF

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产品型号：abs00877
厂商性质：生产厂家
更新时间：2024-07-07
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细胞生物学

重组蛋白原代细胞分离细胞培养细胞检测细胞/组织处理外泌体研究其它动物血清 3D/类器官培养

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详细介绍

品牌	absin	货号	abs00877
规格	10ug	供货周期	一周

概述
种属	Human
形态	Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
来源	Escherichia coli.
氨基酸序列	MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
内毒素水平	Less than 1 EU/µg of Recombinant HumanbFGF as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.
Accession #	P09038
预期分子量	Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of less than 0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度	> 96 % by SDS-PAGE and HPLC analyses.
Gene IDs	2247

参考文献
1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6.<br/>2. Gospodarowicz D. 1974. Nature. 249:123-7.<br/>3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.<br/>4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.<br/>5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57.<br/>6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29.<br/>7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

参考文献

1. Armelin HA. 1973. Proc Natl Acad Sci U S A. 70:2702-6. 2. Gospodarowicz D. 1974. Nature. 249:123-7. 3. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49. 4. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 5. Landriscina M, Bagala C, Mandinova A, et al. 2001. J Biol Chem. 276:25549-57. 6. Fernandez IS, Cuevas P, Angulo J, et al. 2010. J Biol Chem. 285:11714-29. 7. Liu Y, Song Z, Zhao Y, et al. 2006. Biochem Biophys Res Commun. 346:131-9.

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Recombinant Human bFGF

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