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| 品牌 | absin | 货号 | abs00994 |
|---|---|---|---|
| 规格 | 5ug | 供货周期 | 一周 |
| 概述 | |
|---|---|
| 种属 | Murine |
| 形态 | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| 来源 | Escherichia coli. |
| 氨基酸序列 | APTRSPITVT RPWKHVEAIK EALNLLDDMP VTLNEEVEVV SNEFSFKKLT CVQTRLKIFE QGLRGNFTKL KGALNMTASY YQTYCPPTPE TDCETQVTTY ADFIDSLKTF LTDIPFECKK PGQK |
| 内毒素水平 | Less than 1 EU/μg of rMuGM-CSF as determined by LAL method. |
| 活性 | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine FDC-P1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
| Accession # | P01587 |
| 预期分子量 | Recombinant murine GM-CSF is a 14.1 kDa globular protein consisting of 124 amino acids residues. |
| 性能 | |
|---|---|
| 溶解方法 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| 保存方法 | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 纯度 | > 98 % by SDS-PAGE and HPLC analyses. |
| Gene IDs | 12981 |
| 参考文献 | |
|---|---|
| 1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.<br/>2. 1989. N Engl J Med, 320: 253-4.<br/>3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.<br/>4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.<br/>5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31. | |
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