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Recombinant Mouse M-CSF(2ug)

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  • 产品型号:abs00996
  • 厂商性质:生产厂家
  • 更新时间:2024-07-08
  • 访  问  量:983

详细介绍

品牌absin货号abs00996
规格2ug供货周期一周
概述
种属Murine
形态Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.
来源Escherichia coli.
氨基酸序列KEVSEHCSHM IGNGHLKVLLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE
内毒素水平Less than 1 EU/μg of rMuM-CSF as determined by LAL method.
活性Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
Accession #Q3U4F9
预期分子量Approximay 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.
性能
溶解方法We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
纯度> 95 % by SDS-PAGE and HPLC analyses.
参考文献
1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.<br/>2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.<br/>3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.<br/>4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.

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