全国服务咨询热线:

Products产品中心
首页 > 产品中心 > 细胞生物学 > 重组蛋白 > abs04188Recombinant Human Sonic Hedgehog(10ug)

Recombinant Human Sonic Hedgehog(10ug)

简要描述:AG8亚国服务特色产品线(全部现货):
二抗、标签抗体、对照抗体;WB相关:ECL发光液、预染marker、预制胶;IHC相关:二抗试剂盒、组化笔;IP/CoIP试剂盒;细胞因子、信号通路调节剂;血清、BSA、蛋白酶K、CTB、TTX、CEE;凋亡试剂盒、呼吸爆发试剂盒;动植物提取物...

  • 产品型号:abs04188
  • 厂商性质:生产厂家
  • 更新时间:2024-07-08
  • 访  问  量:1148

详细介绍

品牌absin货号abs04188
规格10ug供货周期一周
概述
描述Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.
别名Sonic Hedgehog Protein, SHH, HHG-1
形态Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.
来源E.coli
氨基酸序列MCGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKISRNSERFKELTPNYNPDIIFK DEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSD RDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG
内毒素水平Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
Accession #Q15465
性能
背景Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.
溶解方法Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存方法Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
纯度Greater than 95% as determined by reducing SDS-PAGE.

产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
AG8亚国服务(上海)生物科技有限公司
地址:上海市浦东新区新浩路58号申江科创园18栋
邮箱:wulan@absin.cn
传真:
关注我们
欢迎您关注我们的微信公众号了解更多信息:
欢迎您关注我们的微信公众号
了解更多信息